OPTIMIZATION OF ENZYME ACTIVITY OF L-ASPARAGINASE DERIVED FROM Enterobacter agglomerans SB 221 BACTERIAL SYMBIONT OF BROWN ALGAE Sargassum sp.

Abstract

L-asparaginase an enzyme belonging to the class of hydrolases which enable hydrolyze L-Asparagine to L-aspartic acid and ammonia is used in leukemia cancer treatment. L-asparaginase was purified from Enterobacter agglomerans SB 221, epiphytic bacterial symbiont of brown algae, Sargassum sp. This research aimed to optimize the concentration of L-Asn substrate, fermentation time, and CoCl2 concentration for high enzyme production. Also, the optimum pH, temperature, and CoCl2 concentration for the most efficient enzymatic activity were determined. Nessler Method determined the enzymatic activity of L-asparaginase.